SignalChemDx CN

ORDER SUPPORT

SignalChem places a great deal of emphasis on delivering high quality research related products and services to enable your discovery efforts. Should you have a general inquiry about our products or services, we would be pleased to assist you.

Prices

Prices quoted on SignalChem’s website are expressed in US dollars and apply to territories where SignalChem sells direct. Please note that the prices are subject to change without notification.

If you are located outside of these areas, please liaise with the distributor within your territory for pricing and ordering.
If a price discrepancy exists between the quoted price on the website and the actual price, SignalChem will contact you prior to shipping your order. Should the list price quoted on the website be lower than the actual price of the product, SignalChem will charge the lower price.

Order

For direct-end users, the following options are available:

1. For credit card payment, please use our Online store. (Please see Online Orders and Payments for more information)
2. For payment using purchase order, please email orders@signalchem.com and we will get back to you
3. Fax - please send to +1 604-232-4601
4. Phone - please call 1-866-9-KINASE (54-6273)

Be sure to include any special shipping instructions along with your order. Order confirmations will be sent electronically to the specified email provided at the time of purchase.

For Distributors please click here

Online Orders and Payments:

If you are a new customer, we require you to open a new account - click here to register.

If you already have an account, log in and select the products you wish to purchase, add promo code (if any) and check out accordingly.

SignalChem accepts Visa and Mastercard only. All sales are F.O.B. from point of shipment, pre-paid and added to the invoice.

Bulk Orders

All products supplied by SignalChem are available for purchase in bulk quantities or as custom preparations. Should you require our products to be supplied in different pack sizes to meet you specific research requirements, please contact us at info@signalchem.com or fill out our Inquiry Form and a sales representative will get back to you within one (1) business day.

Shipping Terms

All orders to be shipped within North America, which are received by 10:00 AM PST (1:00 PM EST) Monday to Wednesday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. International orders are shipped on Fridays only. Freight charges may include, but are not limited to priority service and packaging materials. SignalChem is not responsible for any fees that may be incurred by your order such as brokerage costs, duties and taxes. (View FAQ for Shipping and Handling below).

Backorders

SignalChem typically maintains sufficient inventory to meet the needs of its clients and partners. If a product is placed on backorder, SignalChem will notify you of this at the time of your order and will do its best to fill the order within a thirty (30) day period. For items on backorder longer than the specified time interval, SignalChem will contact you to verify your order. Customers will be billed for the order upon shipment.

Conditions of Use

Products sold by SignalChem are all intended for research use only by qualified laboratory personnel. These products are not intended for in vitro diagnostic (IVD) or therapeutic use.

Product Performance Feedback

SignalChem guarantees that all of its products meet the specific requirements set forth within the lot specific technical data sheets (TDS). Should you have a technical issue with one of our products, please contact our technical services group by phone 1-866-9 KINASE (54-6273) or by email: support@signalchem.com with your specific query. If it is determined, solely by SignalChem, that one of our products has failed to conform to the specifications set forth in the TDS, we will be pleased to replace the product free of charge.

Confidentiality

We keep all customer information strictly confidential. All information and/or materials provided by the customer will be used only as directed.

STILL HAVE QUESTIONS?
Please feel free to contact us via our Online Support Form or via Phone or Email
Phone: 1-866-9-KINASE(54-6273) or 1-604-232-4600
Email: support@signalchem.com

SHIPPING & HANDLING FAQs

SignalChem understands the importance of hassle free deliveries. Hence, we place the utmost importance on delivering your products in good condition and on time.

When is my order shipping out?

All orders to be shipped within North America, which are received by 10:00 AM PST Monday to Wednesday, will usually be shipped out on the same day. International orders are shipped on Mondays and Fridays.

How much is the shipping cost?

Freight charges vary depending on the final destination. SignalChem is not responsible for any fees that may be incurred by your order such as brokerage costs, duties and taxes. Please contact us to obtain an estimate.

Do you ship with couriers other than FedEx?

Yes. We also ship with UPS and World Courier.

The products arrived without dry ice. Are they still fit for usage?

To ensure the quality of our product, SignalChem does not recommend customers to use products that arrived without dry ice left in the package. A replacement may be sent under specific circumstances. Please contact us to request a replacement.

STILL HAVE QUESTIONS?
Please feel free to contact us via our Online Support Form or via Phone or Email
Phone: 1-866-9-KINASE(54-6273) or 1-604-232-4600
Email: support@signalchem.com

TECHNICAL SUPPORT FAQs

Quickly find out if we have already addressed your query; click on a question to drop down to the answer.

How do you calculate an enzyme’s purity?

For all of our products we run SDS-PAGE gel with a titration of the enzyme, followed by the densitometry measurement to determine the purity.

Where do I find the sequence of the protein?

To request the sequence information for the protein of interest, please contact us.

How much enzyme should I use for my assay?

An enzyme titration curve and calculated specific activity are included in the data sheet of each active enzyme. It is recommend that the researchers perform a serial dilution of an enzyme product to determine the optimal amount to use in their own assay systems.

How do you calculate a protein’s concentration?

The concentration of each of our products is calculated from a titration on SDS-PAGE gel with a standard curve of the Bovine serum albumin (BSA) or the Bovine gamma globulin (BGG).

Do you offer the enzymes at a higher concentration?

Yes, we may provide enzymes at a higher concentration. Please contact us to obtain more detailed information.

Can I purchase proteins with different buffer formulation?

Yes, we can supply our protein in a customized buffer formulation. Please contact us to submit your request.

What is the difference between active and unactive proteins?

SignalChem’s active enzymes are produced using specific treatment or conditions to achieve optimal activity in vitro, while unactive proteins are generally produced without any stimulation or from prokaryotic systems so the enzymes only show minimal basal activity. Active enzymes can be directly used in biochemical assays for compound screening or enzymatic studies. Unactive proteins can be used as substrates, negative controls, or to study activation of the particular enzymes in vitro.

How are your enzymes activated?

SignalChem’s active enzymes are activated using specific protocols during the process of expression and production to achieve optimal post-translation modification and in vitro activity. The treatment methods may vary for different enzymes depending on their functional roles.

Do you express proteins with any tags other than HIS and GST?

Yes, we do customize the proteins with other tags (e.g. FLAG, Fc). Please contact us for the detailed information.

Do you offer untagged proteins?

Yes, we can customize the protein as per your requirements. Please contact us to submit your query.

How do I find information on the tag cleavage site for my protein?

Please contact us to obtain the details regarding tag cleavage site.

Why are some of your recombinant proteins expressed in E.coli cells while others in Sf9 insect cells?

Expression system is chosen based on the function and application of each product.

Are your enzymes suitable for crystallography studies?

SignalChem’s active enzymes are validated for in vitro activity assays. We offer a wide range of custom services including additional purification steps, buffer exchange, and tag modification to fulfill the requirements for other applications such as protein crystallography. Please contact us to find more information about this service.

I want to use the protein for binding studies. Which protein type do you recommend – Active Enzyme or Recombinant Protein?

SignalChem’s active enzymes and recombinant proteins both may work in binding assays. We recommend clients to test the compatibility of a product in their assay system. Contact us to request a free sample.

Do you determine the Km values of the protein?

SignalChem offers Profiling Services for enzymatic studies including Km determination. Please click here to find more information about this service.

What is the level of phosphorylation of your Active Kinases?

Generally active kinases are phosphorylated on certain sites in their catalytic domain. However, the number of total phosphorylation sites from each active kinase may vary. We do not perform any test to probe for specific phosphorylation in our kinases.

How do I know whether or not certain key residues in the protein are phosphorylated?

Phosphorylation of a certain residue can be determined by Western Blot or ELISA using phospho-specific antibodies.

STILL HAVE QUESTIONS?
Please feel free to contact us via our Online Support Form or via Phone or Email
Phone: 1-866-9-KINASE(54-6273) or 1-604-232-4600
Email: support@signalchem.com

TECHNICAL RESOURCES

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(Negative)-Regulation of Cyclin-Dependent Kinases

The cell cycle is controlled by a series of checkpoints which when bypassed, enable the cell to proceed with division and replication. Cyclin-dependent kinases (CDKs) are the primary regulators of the cell cycle. CDKs are small with much of the peptide sequence representing its protein kinase domain; therefore, CDKs rely on interactions with cyclin regulatory subunits for enzyme activation and target specificity. Cyclins achieve temporal control of CDK function via fluctuations in their synthesis and degradation.

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