Recombinant Aspergillus fumigatus allergen Asp f 5 was expressed in E. coli using a C-terminal His tag.
AF05-A931H-250 |
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基因别名:
Extracellular metalloproteinase mep, AfuMep, Allergen Asp f 5, Elastinolytic metalloproteinase mep, Fungalysin mep
Genbank编号:
参考文献:
1. Sirakova TD, Markaryan A, Kolattukudy PE. Molecular cloning and sequencing of the cDNA and gene for a novel elastinolytic metalloproteinase from Aspergillus fumigatus and its expression in Escherichia coli. Infect Immun. 1994 Oct;62(10):4208-18. doi: 10.1128/iai.62.10.4208-4218.1994. PMID: 7927676; PMCID: PMC303097.
2. Markaryan A, Morozova I, Yu H, Kolattukudy PE. Purification and characterization of an elastinolytic metalloprotease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung. Infect Immun. 1994 Jun;62(6):2149-57. doi: 10.1128/iai.62.6.2149-2157.1994. PMID: 8188335; PMCID: PMC186491.
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