概述:

CRISPR (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development (3). Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. FnCas12a from Francisella tularensis subsp. novicida shows increased stability at >37°C, making it ideal for LAMP (loop-mediated isothermal amplification) applications (4).

Genbank编号：

WP_003040289

参考文献：

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.