概述:

TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).

Genbank编号：

WP_011229148.

参考文献：

1. Niewoehner, O. et al: Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. Rna 22(3):318-329,2016.

2. Niewoehner, O. et al: Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548, 2017.