概述:

PureScript III Pro Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H ) Reverse Transcriptase. Compared with the previous generation, PureScript III Pro Reverse Transcriptase is designed for efficient reverse transcription reactions at 37oC, while retaining the thermostability of PureScript II Pro. For RNA with complex secondary structure, the reverse transcription reaction temperature can be raised to 50 ~ 55 oC, avoiding the inhibitory effect of complex secondary structure on cDNA synthesis. In addition, this product still has superior continuous synthesis ability and superior impurity tolerance. It is applicable for reverse transcription of animal, plant, and microbial RNA.

参考文献：

1. Kotewicz ML, D'Alessio JM, Driftmier KM, Blodgett KP, Gerard GF. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35(3):249-58. PMID: 2412939.
2. Gerard GF, D'Alessio JM, Kotewicz ML, Noon MC. Influence on stability in Escherichia coli of the carboxy-terminal structure of cloned Moloney murine leukemia virus reverse transcriptase. DNA. 1986 Aug;5(4):271-9. PMID: 2427293.