概述:

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. It has a point mutation which leads to removal of RNase activity. Compared to other M-MLV mutants that only have the pol gene of M-MLV, M-MLV (H-) retains complete protein structure and polymerase activity. It can be used for synthesis of longer cDNA or preparation of cDNA library.

参考文献：

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