概述:

Taq DNA polymerase is a highly thermostable DNA polymerase of Thermus aquaticus. The enzyme exhibits 5’→3’ polymerase activity and possesses 5’→3’ exonuclease activity but has no detectable 3’→5’ exonuclease activity. In addition, Taq DNA polymerase generates PCR products containing extra adenines at the 3’-ends. The resulting products can be used for TA cloning. Applications of this product include: routine PCR amplification of DNA fragments, generation of PCR products for TA cloning, DNA labelling and DNA sequencing.

参考文献：

1. Innis M A, et al: PCR protocols and applications: A laboratory manual, academic, New York, 1989.
2. Innis M A, et al: DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Proc. Natl. Acad. Sci. USA. 1988, 85:9436-9440.
3. Weyant R S, et al: Effect of ionic and nonionic detergents on the Taq polymerase. Biotechniques. 1990, 9:308-309.